By Hiroshi Nakato, Susumu Izumi, Scott B. Selleck (auth.), Y. Kitagawa, T. Matsuda, S. Iijima (eds.)
Animal mobilephone expertise is a growing to be self-discipline of mobile biology which goals not just to appreciate constructions, features and behaviors of differentiated animal cells but in addition to envision their talents for use for commercial and clinical reasons. The aim of animal mobilephone know-how contains accomplishments of clonal enlargement of differentiated cells with helpful skill, optimization in their tradition stipulations, modulation in their skill for creation of medically and pharmaceutically vital proteins, and the appliance of animal cells to gene remedy and synthetic organs. This quantity offers the readers a whole evaluate of the current cutting-edge in Japan. The court cases can be important for mobile biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines with regards to animal telephone tradition, operating both in educational environments or in industries of biotechnology and pharmacy.
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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Tenth Annual Meeting of the Japanese Association for Animal Cell Technology, Nagoya, Japan, November 5–8, 1997 Volume 10
The results suggest that CTL are continuously expandable when cultured on formalin-fixed autologous tumor cells as well as when cultured on live cells of an autologous established tumor cell line. Even though we have failed to establish an autologous tumor cell line, many investigators may have target tumor cells available found in small pieces of dissected tumor tissue, in the fluid of the thoracic cavity and ascites, or in quiescent primary cultures. If fixed and supplied properly, these cells could also serve as a source of tumor antigens available any time for further stimulation of CTL .
C. Roche. (1991) Characterization and isolation of an intracellular mannose specific receptor from the human promyelocytic HL60 cells. Carbohydr. Res. 213, 95-108. Privat, J. , F. Delmotte, and M. Monsigny. (1974) Protein-sugar interaction. Association of linked N-acetyl-D-glucosamine oligomer derivatives with wheat germ agglutinin (lectin). FEES Lett. 46, 224-228. , S. Bourgerie, N. Normand-Sdiqui, R. Mayer, G. Strecker, A. C. Roche, and M. Monsigny. (1998) Novel glycosynthons for glycoconjugate preparation : oligosaccharyl-pyroglutamyl-anilide derivatives.
1. 1. Expression Cloning of Two Types of cDNAs We constructed an expression cloning system using both an Epstein-Barr virus-based cloning vector pAMo and Burkitt lymphoma cell line Namalwa KJM-1 as a host. To isolate glycosyltransferases involved in the biosynthesis of the determinants, we developed an expression cloning system using lectin-resistance selection. Ricinus communis agglutinin is a cytotoxic lectin specific to galactose residues, especially structure, which is a backbone of the structure.